embryo fibroblast meaning in Chinese
胚胎成纤维细胞
Examples
- Culture of the attenuated a66 strain of duck hepatitis virus in chicken embryo fibroblast
66弱毒株在鸡胚成纤维细胞上的培养 - Development of chicken embryo fibroblast cell line resistant to j subgroup avian leukosis virus infection
亚群禽白血病病毒的鸡胚成纤维细胞系建立 - Construction of recombinant fowlpox virus coexpressing aiv ha and ndv f . for the construction of transfer vector pfgs11haf , aiv ha gene of f strain in puc18ha and ndv f gene of f48e8 strain in puc19f were removed and inserted into pfgs11 . recombinant fowlpox viruses ( rfpv ) coexpressing aiv ha gene and ndv f gene were constructed by using different promoters of ps and pe / l . recombinant rfpvs were derived by dosper liposome - mediated transfection with the two transfer vectors on chicken embryo fibroblast ( cef ) monolayer cultures which were infected by wild type fpv chinese vaccine strain 282e4 3 - 4 hours earlier
Puc18ha和sk质粒同时经hind 、 kpn酶切后连接得中间质粒skha ；将质粒skha用bamhi酶切回收ha基因插入到插入载体pfgs11中的bamhi位点，通过酶切鉴定获得了pfgs11ha ；将含ndvf基因的质粒puc19f用hind 、 sal酶切经klenow酶补平插入到经sma酶切后的skifn中pe / l启动子下获得中间质粒skf ，再将质粒skf和puc18质粒先分别用ecor 、 xho酶切klenow补平，后再共同用sac酶切连接得puc18pelf ， sal酶切回收pe / l - f基因盒插入到pfgs11ha的sal位点，通过酶切鉴定获得了pe / l - f与ps - ha同向的表达载体pfgs11haf 。 - Meq protein , highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro . the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )
利用通过杆状病毒载体在昆虫细胞系sfg上高度表达的meq蛋白产物免疫balb / c小鼠，然后收获其免疫脾细胞并与肿瘤细胞系spz / 0通过peg于体外融合;获得的杂交瘤细胞被克隆并通过与mdv感染的鸡胚成纤维细胞( cef )做免疫荧光试验( fa ) ，进行其分泌抗meq单克隆抗体( mcab )能力的筛选。 - From dead chicken which infected infectious stunting syndrom of our province , one virue was isolated using spfeggs , chicken embryo fibroblast , mdck18 , and vero cell . this virus was unable to agglutinate chicken erythrocytes . in order to definite the pathogeny of infectious stunting syndrom . physical and chemical specific property , types of the nucleic acid of the isolated virus , recurrent infection and other biological property determination and indirect elisa test proved it as a parvoviruses like strain of chicken
为确定该病的病原，对所分离病毒进行了理化特性测定、病毒核酸型别测定、动物回归试验等生物学特性测定，证明该分离病毒与细小病毒科( parvovirdae )细小病毒属( parvovirus )的鸡细小病毒( chickenparvovirus )特性基本相符，核酸型为dna型。